Nicotine effect toward the oocyte level of rats (Rattus novergicus)

Objective: To discover the effect of some nicotine's doses to the maturity of rat's oocyte
in reproductive age.
Methods: This study was using an experimental design with 3 treatment groups (subcutaneous
injection of nicotine 35, 52.5 and 70 mg/kgBW/d for 7 d) and 1 control group.
After nicotine exposure treatment, the oocytes of rats were taken for the histological
purpose to observe the oocyte's maturity grade in germinal vesicle (GV), Germinal
Vesicle Breakdown (GVBD), Metaphase I (M I), or Metaphase II (M II). All of the
oocytes were stained using Aceto orcein 1%. The data was analyzed based on One-Way
Analysis of Variance (a = 0.05).
Results: The significant result got further analyzed using LSD test. The result obtained
that there were no significant differences of GV and GVBD oocytes in all groups.
Otherwise, the result of One-Way ANOVA showed that there was significant differences
of M−II oocytes in all groups. LSD test obtained that there was significant differences
between control group and 3 treatment groups, and nicotine's treatment 52.5 mg/kg body
weight (BW) with 35 mg/kg BW. This research obtained that the number of GV and
GVBD oocytes were lower than the control group.
Conclusion: There was no M I and M II oocytes, because nicotine treatment were less
than the control group.