Protection of Centella asiatica Extract Through BDNF Expression on Stunting Model Zebrafish Larvae (Danio rerio) by Rotenone Induced

Ridlayanti, Annisa, Wijayanti, Aida Ratna, Khotimah, Husnul and Ali, Muljohadi Protection of Centella asiatica Extract Through BDNF Expression on Stunting Model Zebrafish Larvae (Danio rerio) by Rotenone Induced. In: Proceedings of the 1st Paris Van Java International Seminar on Health, Economics, Social Science and Humanities (PVJ-ISHESSH 2020), 8 March 2021, Bandung.

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Abstract

Objectives: Stunting in WHO Child Growth
Standards is based on the length-for-age indexes with less than
-2 SD of z score. Rotenone is a pesticide model that acts as an
mitochondrial complex I inhibitor and Endocrine Disrupting
Chemicals (EDCs). Brain Derived Neurotrophic Factor
(BDNF) is one of growth factors mostly found in central
nervous system and slightly in peripheral nerves. One of
natural herb, Centella asiatica, has antioxidant effects and
grows in tropical areas in Indonesia. The aim of this research
was to know the effects of Centella asiatica extracts on
zebrafish larvae (Danio rerio) by Rotenone induced, through
Brain Derived Neurotropic Factor (BDNF) expression.
Methods: Zebrafish embryos were divided into five groups
including control (C), rotenone group (R), and Centella
asiatica group (Ca1;Ca2;Ca3) with concentration of the each
2.5, 5, and 10 g/mL. The entire groups had been started since
2 until 72 hpf (half post fertilization) and followed its
development up to the age of larvae 9 dpf (days post
fertilization). It was followed by measuring the body length at
the age of 3, 6 and 9 dpf using Image Raster software. After
termination zebrafish larvae, the BDNF expression
measurement was measure by using an
Immunohistochemistry (IHC) wholemount method with
diamino benzidine (DAB). Brown colour density values was
quantified by using Image J software. Results : There was a
significant difference in the mean of body length which was
shown by Ca2 group (5 g/ml) compared with rotenone group
(p < 0,05). The BDNF expression increased in all three
Centella asiatica groups compared with rotenone group.
However, only Centella asiatica group (5 g/ml) showed the
most significant effect on the increase of BDNF expression (p
<0.05). Conclusion: Protection of Centella asiatica extract
increases the body length and BDNF expression on the
stunting model zebrafish larvae induced by rotenone.

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	Centella asiatica, rotenone, zebrafish, stunting, body length, BDNF
Subjects:	R Medicine > RG Midwifery
Divisions:	Faculty of Health Sciences > Department of Midwifery
Depositing User:	Library Umpo
Date Deposited:	07 Apr 2021 05:10
Last Modified:	03 Jun 2021 03:24
URI:	https://eprints.umpo.ac.id/id/eprint/6419

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